EP3056563

• Healios K.K; Riken; Osaka Universitysince Jul 15, 2016; EPO record ends Apr 30, 2021

Provided are a production method of retinal pigment epithelial cell that improves differentiation induction efficiency of pluripotent stem cell into retinal pigment epithelial cell, and can provide highly pure retinal pigment epithelial cell by a simple and easy operation in a short period, a culture method of retinal pigment epithelial cell that can stably grow and culture a cell, a toxicity/efficacy evaluation method using a retinal pigment epithelial cell useful for transplantation therapy, and a therapeutic drug for a retinal disease. The present invention relates to a production method of retinal pigment epithelial cells, including a step of adhesion culture of human pluripotent stem cells by using a culture substrate coated with a laminin-E8 fragment, a culture method of retinal pigment epithelial cells, including a step of adhesion culture of retinal pigment epithelial cells by using a culture substrate coated with a laminin-E8 fragment, a toxicity or efficacy evaluation method using retinal pigment epithelial cells obtained by producing or culturing by said method, and a therapeutic drug for a retinal disease, containing the retinal pigment epithelial cells.